RESUMO
Land-use conversion changes soil properties and their microbial communities, which, combined with the overuse of antibiotics in human and animal health, promotes the expansion of the soil resistome. In this context, we aimed to profile the resistome and the microbiota of soils under different land practices. We collected eight soil samples from different locations in the countryside of São Paulo (Brazil), assessed the community profiles based on 16S rRNA sequencing, and analyzed the soil metagenomes based on shotgun sequencing. We found differences in the communities' structures and their dynamics that were correlated with land practices, such as the dominance of Staphylococcus and Bacillus genera in agriculture fields. Additionally, we surveyed the abundance and diversity of antibiotic resistance genes (ARGs) and virulence factors (VFs) across studied soils, observing a higher presence and homogeneity of the vanRO gene in livestock soils. Moreover, three ß-lactamases were identified in orchard and urban square soils. Together, our findings reinforce the importance and urgency of AMR surveillance in the environment, especially in soils undergoing deep land-use transformations, providing an initial exploration under the One Health approach of environmental levels of resistance and profiling soil communities.
RESUMO
This study aimed to evaluate the influence of capsaicin and ascorbic acid on the physiology of biofilm formation and dispersion. The influence of the E. coli ATCC 25922 biofilm and five E. coli isolates were observed in the presence of sodium hypochlorite 2.5 mM and subinhibitory concentrations of capsaicin (7 mM) and ascorbic acid (100 mM). The cells counts were performed through standard plaque count and the architecture visualized by confocal microscopy. The proteins, carbohydrates, and DNA present in the biofilm matrix were also quantified. There was a reduction in adhered cells in the presence of capsaicin (7 mM) and ascorbic acid (100 mM) in the biofilm formation kinetics, however, regarding the dispersion, only capsaicin altered the biofilm, data confirmed by confocal fluorescence microscopy (p < 0.05). It was noted that the matrix composition is dynamic and may be affected by changes in growth conditions such as the presence of antimicrobial substances.
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INTRODUCTION: Escherichia coli is a Gram-negative opportunistic human pathogen, which has aroused considerable medical interest for being involved in cases of urinary tract infection. AIM: Characterize the E. coli isolated both in the hospital and in the community. METHODOLOGY: A total of 200 E. coli isolated in urine samples from hospital and community were evaluated in biofilm formation assay and hydrophobicity MATS method. Antimicrobial susceptibility was performed through agar-diffusion technique. Virulence and ESBL production genes were observed through the polymerase chain reaction amplification of papC, fimH, fliC, kpsMTII, blaTEM, blaCTX-M, blaSHV , and blaOXA. The phylogenetic classification was based on the pattern chuA and yjaA and the region TspE4.C2 by PCR Multiplex. RESULTS: A higher frequency of non-adherent or poorly adherent isolates was observed in the community group. Approximately 85% of the community isolates were distributed in the highest hydrophilicity group (p<0.05). The level of resistant microorganisms was present at the same level in both source (p>0.05). About 14% of the hospital isolates were positive in the ESBL phenotypic detection test (p>0.05). Among the samples, 95% presented ESBL-encoding genes. The predominant phylogenetic group was B2 (78%). Community isolates showed a higher prevalence of virulence genes fimH, papC, and kpsMTII when compared to hospital samples. CONCLUSION: These data confirm the worldwide trend that isolates in the community present sometimes higher levels of virulence and antimicrobial resistance.
